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KMID : 0903619960370010118
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 1 p.118 ~ p.122
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Effect of Different Sealing Materials on CO2 and Ethylene concentration in Culture Vessel , and Growth and Vitrification of Gypsophila paniculata ´ Bristol Fairy ´
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Abstract
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CO₂ and ethylene production of Gypsophila paniculata `Bristol Fairy´ in shoot cultures with different sealing materials were investigated. The concentration of CO₂ released from vitrified plantlets was higher than those of normal ones. However both of them had ability to photosynthesis, even in different CO₂ concentrations between in dark and in light. The vitrified plantlets released less ethylene. CO₂ and ethylene concentrations in culture vessel over normal plantlets sealed with aluminium foil increased until 4 days after culture. As time had passed, vitrification of normal plantlets increased. These results suggested that respiration of plantlets increased with plant growth causing increased vitrification of normal plantlets and increased concentration of ethylene in culture vessels. The vitrification percentage was 56.1 in plantlets sealed aluminium foil where that was 2.5% in plantlets sealed with Milli wrap that could pass through air. There were, however, no significant differences in number of shoots. Vitrification percentage increased continuously in plantlets sealed with aluminium foil as subculture go on, but decreased to less than 10% in those sealed with uni wrap and filter paper that could pass through air. But vitrification percentage in plantlets sealed with Milli wrap was very low regardless of subcultures.
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